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| カタログ番号 | HW557014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human HINT1 Polyclonal Antibody (HW557014) is a rabbit polyclonal antibody detecting HINT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Pongo abelii, and Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human HINT1 (Met1-Gly126). | ||||||||
| ターゲット | Histidine triad nucleotide-binding protein 1, HINT, Adenosine 5'-monophosphoramidase, HINT1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P49773 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Adenosine 5'-monophosphoramidase HINT1 is a ~13 kDa protein. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. 1. Chou, TF. et al. (2005) The Journal of biological chemistry 280, 15356-61. PMID: 15703176 2. Weiske, J. et al. (2006) The Journal of biological chemistry 281, 27356-66. PMID: 16835243 3. Chou, TF. et al. (2007) Molecular pharmaceutics 4, 208-17. PMID: 17217311 4. Chou, TF. et al. (2007) The Journal of biological chemistry 282, 15137-47. PMID: 17337452 5. Wang, J. et al. (2012) The journal of physical chemistry. B 116, 6798-805. PMID: 22329685 6. Zhou, X. et al. (2013) Biochemistry 52, 3588-600. PMID: 23614568 7. Shah, R. et al. (2017) Biochemistry 56, 3559-3570. PMID: 28691797 8. Shah, RM. et al. (2018) Journal of molecular biology 430, 2709-2721. PMID: 29787766 9. Strom, A. et al. (2020) FEBS letters 594, 1497-1505. PMID: 31990367 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-HINT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: K562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Hela cell lysate
Lane 5: HepG2 cell lysate
Lane 6: MCF-7 cell lysate
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