Anti-Human HLA-DRA Recombinant Antibody (1D09C3) (HM812107)

Name: Anti-Human HLA-DRA Recombinant Antibody (1D09C3)
Brand: abinScience
SKU: HM812107
Price: 118 USD
Availability: InStock

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Обзор

Каталожный номер	HM812107
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG1, kappa
ID клона	1D09C3
Цель	HLA-DRA1, MHC class II antigen DRA, HLA-DRA, HLA class II histocompatibility antigen, DR alpha chain
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P01903
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA) is a ~28 kDa protein. An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. 1. Shams, H. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 1966-77. PMID: 15265931 2. Röhn, TA. et al. (2004) Nature immunology 5, 909-18. PMID: 15322540 3. Deng, L. et al. (2007) Nature immunology 8, 398-408. PMID: 17334368 4. Kwok, WW. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 188, 2537-44. PMID: 22327072 5. Scally, SW. et al. (2013) The Journal of experimental medicine 210, 2569-82. PMID: 24190431 6. Muehling, LM. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 197, 3214-3224. PMID: 27591323 7. Galperin, M. et al. (2018) Science immunology 3. PMID: 29884618 8. Abelin, JG. et al. (2019) Immunity 51, 766-779.e17. PMID: 31495665 9. Stern, LJ. et al. (1994) Nature 368, 215-21. PMID: 8145819 10. Kropshofer, H. et al. (1997) Immunity 6, 293-302. PMID: 9075930
Примечание	For research use only.

Изображения

Bioactivity

SEC-HPLC detection for Anti-Human HLA-DRA Antibody (1D09C3).
Flow Cytometry

Flow-cytometry using anti-human HLA-DRA antibody. Human U-87 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human HLA-DRA antibody monoclonal antibody (Catalog # HM812107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.