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Anti-Human HNRNPL Polyclonal Antibody (HB914014)

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Aperçu
Numéro de catalogueHB914014
Description
Anti-Human HNRNPL Polyclonal Antibody (HB914014) is a rabbit polyclonal antibody detecting HNRNPL in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human HNRNPL (Ser381-Ser589).
Cible hnRNP L, HNRPL, Heterogeneous nuclear ribonucleoprotein L, HNRNPL
Purification Purified by antigen affinity column.
Numéro d'accès P14866
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Heterogeneous nuclear ribonucleoprotein L (HNRNPL) is a ~64 kDa protein. Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPK and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation.

1. Kuninger, DT. et al. (2002) Nucleic acids research 30, 823-9. PMID: 11809897
2. Liu, G. et al. (2012) The Journal of biological chemistry 287, 22709-16. PMID: 22570490
3. Shankarling, G. et al. (2014) Molecular and cellular biology 34, 71-83. PMID: 24164894
4. Loh, TJ. et al. (2015) Biochimica et biophysica acta 1849, 743-50. PMID: 25623890
5. Blatter, M. et al. (2015) Journal of molecular biology 427, 3001-22. PMID: 26051023
6. Piñol-Roma, S. et al. (1989) The Journal of cell biology 109, 2575-87. PMID: 2687284
7. Hollensen, AK. et al. (2020) eLife 9. PMID: 33174841
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human HNRNPL Polyclonal Antibody

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