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| カタログ番号 | HD661014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human HTATSF1 Polyclonal Antibody (HD661014) is a rabbit polyclonal antibody detecting HTATSF1. Suitable for Mouse and Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human HTATSF1 (Phe125-Ser389). | ||||||||
| ターゲット | 17S U2 SnRNP complex component HTATSF1, HIV Tat-specific factor 1, HTATSF1, Tat-SF1 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O43719 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 背景 | 17S U2 SnRNP complex component HTATSF1 is a ~85 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint-interacting stem loop. HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences. Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation. 1. Loerch, S. et al. (2019) The Journal of biological chemistry 294, 2892-2902. PMID: 30567737 2. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006 3. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310 4. Kim, JB. et al. (1999) Molecular and cellular biology 19, 5960-8. PMID: 10454543 | ||||||||
| 注意事項 | For research use only |
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