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Anti-Human IDH1-R132H-specific Recombinant Antibody (HMab-1) (HF781043)

Anti-Human IDH1-R132H-specific Recombinant Antibody (HMab-1)
Anti-Human IDH1-R132H-specific Recombinant Antibody (HMab-1)
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  • 100ug
  • 1mg
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Aperçu
Numéro de catalogueHF781043
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneHMab-1
Cible IDH1, IDH, NADP(+)-specific ICDH, Isocitrate dehydrogenase [NADP] cytoplasmic, PICD, IDP, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès O75874
RRID Anti-Human IDH1-R132H-specific Antibody (HMab-1) (abinScience Cat# HF781043, RRID:AB_3729711)
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1-R132H-specific) is a ~46 kDa protein. Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.

1. Geisbrecht, BV. et al. (1999) The Journal of biological chemistry 274, 30527-33. PMID: 10521434
2. Dang, L. et al. (2009) Nature 462, 739-44. PMID: 19935646
Note For research use only.
Images
  • Anti-Human IDH1-R132H-specific Recombinant Antibody (HMab-1)

    SDS-PAGE

    SDS-PAGE for Anti-Human IDH1-R132H-specific Antibody (HMab-1)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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