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Anti-Human IFT20 Polyclonal Antibody (HP142014)

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Vista general
Número de catálogoHP142014
Descripción
Anti-Human IFT20 Polyclonal Antibody (HP142014) is a rabbit polyclonal antibody detecting IFT20 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Xenopus laevis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human IFT20 (Met1-Lys132).
Objetivo hIFT20, IFT20, Intraflagellar transport protein 20 homolog
Purificación Purified by antigen affinity column.
Número de acceso Q8IY31
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Intraflagellar transport protein 20 homolog (IFT20) is a ~15 kDa protein. Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling. Essential for male fertility.

1. Pugacheva, EN. et al. (2007) Cell 129, 1351-63. PMID: 17604723
2. Follit, JA. et al. (2006) Molecular biology of the cell 17, 3781-92. PMID: 16775004
3. Schmid, FM. et al. (2018) The Journal of cell biology 217, 151-161. PMID: 29237719
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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