Anti-Human IKBKB Polyclonal Antibody (HT056014)

Name: Anti-Human IKBKB Polyclonal Antibody
Brand: abinScience
SKU: HT056014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HT056014

Descripción

Anti-Human IKBKB Polyclonal Antibody (HT056014) is a rabbit polyclonal antibody detecting IKBKB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human IKBKB (Glu16-Gly345).

Objetivo

IKKB, IKK-beta, Serine/threonine protein kinase IKBKB, I-kappa-B-kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, IKBKB, IKK-B, NFKBIKB, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, IkBKB

Purificación

Purified by antigen affinity column.

Número de acceso

O14920

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Inhibitor of nuclear factor kappa-B kinase subunit beta (IKBKB) is a ~86 kDa protein. Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.

1. Cui, J. et al. (2010) Cell 141, 483-96. PMID: 20434986

2. Tsuchiya, Y. et al. (2010) Molecular cell 39, 570-82. PMID: 20797629

3. Clark, K. et al. (2011) The Biochemical journal 434, 93-104. PMID: 21138416

4. Cardinez, C. et al. (2018) The Journal of experimental medicine 215, 2715-2724. PMID: 30337470

5. Mercurio, F. et al. (1997) Science (New York, N.Y.) 278, 860-6. PMID: 9346484

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-IKBKB polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

Referencias

Datasheet

Anti-Human IKBKB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo