Anti-Human IL-10 Recombinant Antibody (hum12G8) (HB997013)

Name: Anti-Human IL-10 Recombinant Antibody (hum12G8)
Brand: abinScience
SKU: HB997013
Price: 110 USD
Availability: InStock

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1mg

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB997013

Reactivité des espèces

Human

Applications

Blocking, ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG1

ID de clone

hum12G8

Cible

CSIF, Cytokine synthesis inhibitory factor, IL-10, IL10, Interleukin-10

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P22301

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Interleukin-10 (IL10) is a ~20 kDa protein. Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.

1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608

2. El Kasmi, KC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 7215-9. PMID: 18025162

3. Hashimoto, SI. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 3619-25. PMID: 11564774

4. de Waal Malefyt, R. et al. (1991) The Journal of experimental medicine 174, 1209-20. PMID: 1940799

5. Willems, F. et al. (1994) European journal of immunology 24, 1007-9. PMID: 7512027

6. Frei, K. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2720-8. PMID: 8144879

Note

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

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