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| カタログ番号 | HB769023 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, FCM, WB | ||||||
| 宿主種 | Human | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG1, lambda | ||||||
| クローンID | SAA2017 | ||||||
| テスト済みアプリケーション | WB, FCM | ||||||
| ターゲット | IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P29460 | ||||||
| RRID | Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA2017) (abinScience Cat# HB769023, RRID:AB_3727929) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Interleukin-12 subunit beta (IL12B/IL-12 p40) is a ~37 kDa protein. Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. | ||||||
| 注意事項 | For research use only. | ||||||
| Isotype Control | Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107) |
SEC-HPLC detection for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (ABS0390).
Flow-cytometry using anti-human IL12B antibody.IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # HB769023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA2017).
Western blot analysis was performed using anti-IL12B/IL-12 p40/NKSF2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 50ng
Lane 2: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 30ng
Lane 3: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 20ng
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