Anti-Human IL-21 Recombinant Antibody (SAA0390), APC (HV658137)

Name: Anti-Human IL-21 Recombinant Antibody (SAA0390), APC
Brand: abinScience
SKU: HV658137
Price: 256 USD
Availability: InStock

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Numéro de catalogue	HV658137
Reactivité des espèces	Human
Applications	ELISA, FCM
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	SAA0390
Conjugaison	APC
Applications testées	FCM
Cible	IL-21, Interleukin-21, IL21, Za11
Niveau d'endotoxines	Please contact with the lab for this information.
Produits associés	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Numéro d'accès	Q9HBE4
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte	Interleukin-21 (IL21) is a ~18 kDa protein. Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. 1. Parrish-Novak, J. et al. (2000) Nature 408, 57-63. PMID: 11081504 2. Strengell, M. et al. (2004) Journal of leukocyte biology 76, 416-22. PMID: 15178704
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using APC anti-human IL21 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human IL21 monoclonal antibody (Catalog: HV658137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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