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Anti-Human IL-21 Recombinant Antibody (SAA0390), APC (HV658137)

Anti-Human IL-21 Recombinant Antibody (SAA0390), APC
Anti-Human IL-21 Recombinant Antibody (SAA0390), APC
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Спецификация:
  • 50T
  • 100T
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Обзор
Каталожный номерHV658137
Реактивность видовHuman
ПримененияELISA, FCM
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клонаSAA0390
КонъюгацияAPC
Проверенные приложения FCM
Цель IL-21, Interleukin-21, IL21, Za11
Уровень эндотоксинов Please contact with the lab for this information.
Связанные продукты Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Номер доступа Q9HBE4
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

Interleukin-21 (IL21) is a ~18 kDa protein. Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection.

1. Parrish-Novak, J. et al. (2000) Nature 408, 57-63. PMID: 11081504
2. Strengell, M. et al. (2004) Journal of leukocyte biology 76, 416-22. PMID: 15178704
Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Изображения
  • Anti-Human IL-21 Recombinant Antibody (SAA0390), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human IL21 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human IL21 monoclonal antibody (Catalog: HV658137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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