Anti-Human IL‑23 p19 Recombinant Antibody (SAA0392) (HV466207)

Name: Anti-Human IL‑23 p19 Recombinant Antibody (SAA0392)
Brand: abinScience
SKU: HV466207
Price: 196 USD
Availability: InStock

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概要
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データシート
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概要

カタログ番号	HV466207
種反応性	Human
アプリケーション	ELISA, FCM, WB
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG4, kappa
クローンID	SAA0392
テスト済みアプリケーション	WB, FCM
ターゲット	IL-23 subunit alpha, IL-23-A, IL-23p19, IL23A, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, SGRF
エンドトキシンレベル	< 10 EU/mg
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	Q9NPF7
形態	Liquid
保存バッファー	0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	Interleukin-23 subunit alpha (IL23A) is a ~20 kDa protein. Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity. Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4. This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A. In turn, participates in the early and effective intracellular bacterial clearance. Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells. IL23A is the therapeutic target of guselkumab (Tremfya). 1. Oppmann, B. et al. (2000) Immunity 13, 715-25. PMID: 11114383 2. Bloch, Y. et al. (2018) Immunity 48, 45-58.e6. PMID: 29287995 3. Sun, R. et al. (2020) Cellular and molecular gastroenterology and hepatology 10, 673-697. PMID: 32474165 4. Glassman, CR. et al. (2021) Cell 184, 983-999.e24. PMID: 33606986 5. Parham, C. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 5699-708. PMID: 12023369 6. Wilson, NJ. et al. (2007) Nature immunology 8, 950-7. PMID: 17676044
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human IL23A Antibody (ABS0131).
Flow Cytometry

Flow-cytometry using anti-human IL23A antibody.IL23A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL23A antibody monoclonal antibody (Catalog # HV466207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-IL23A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL23A (Catalog No: HV466011)
Lane 2: negative control