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| カタログ番号 | HA984014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human INTS8 Polyclonal Antibody (HA984014) is a rabbit polyclonal antibody detecting INTS8 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human INTS8 (Asn831-Phe995). | ||||||||
| ターゲット | Integrator complex subunit 8, Int8, Protein kaonashi-1, C8orf52, INTS8 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q75QN2 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Integrator complex subunit 8 (INTS8) is a ~113 kDa protein. Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS8 is required for the recruitment of protein phosphatase 2A (PP2A) to transcription pause-release checkpoint. 1. Oegema, R. et al. (2017) PLoS genetics 13, e1006809. PMID: 28542170 2. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860 3. Vervoort, SJ. et al. (2021) Cell 184, 3143-3162.e32. PMID: 34004147 4. Hu, S. et al. (2023) Molecular cell 83, 1588-1604.e5. PMID: 37080207 5. Fianu, I. et al. (2024) Nature 629, 219-227. PMID: 38570683 6. Baillat, D. et al. (2005) Cell 123, 265-76. PMID: 16239144 7. Huang, KL. et al. (2020) Molecular cell 80, 345-358.e9. PMID: 32966759 | ||||||||
| 注意事項 | For research use only. |
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