Anti-Human IPO4 Polyclonal Antibody (HP026014)

Name: Anti-Human IPO4 Polyclonal Antibody
Brand: abinScience
SKU: HP026014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HP026014

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human IPO4 (Asn667-Ser1081).

Objetivo

RanBP4, Imp4b, Importin-4b, Ran-binding protein 4, RANBP4, IPO4, Importin-4, Imp4, IMP4B

Purificación

Purified by antigen affinity column.

Número de acceso

Q8TEX9

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Importin-4 (IPO4) is a ~118 kDa protein. Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

1. Jäkel, S. et al. (2002) The EMBO journal 21, 377-86. PMID: 11823430

2. Miyauchi, Y. et al. (2005) The Journal of biological chemistry 280, 40901-8. PMID: 16207705

3. Pradeepa, MM. et al. (2008) Molecular and cellular biology 28, 4331-41. PMID: 17682055

4. Alvarez, F. et al. (2011) The Journal of biological chemistry 286, 17714-21. PMID: 21454524

5. Apta-Smith, MJ. et al. (2018) The EMBO journal 37. PMID: 30177573

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-IPO4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Lncap cell lysate
Lane 3: A549 cell lysate

Referencias

Datasheet

Anti-Human IPO4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo