Anti-Human IST1 Polyclonal Antibody (HX152014)

Name: Anti-Human IST1 Polyclonal Antibody
Brand: abinScience
SKU: HX152014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HX152014

Description

Anti-Human IST1 Polyclonal Antibody (HX152014) is a rabbit polyclonal antibody detecting IST1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human IST1 (Met1-Glu186).

Cible

Putative MAPK-activating protein PM28, IST1, IST1 homolog, KIAA0174, hIST1

Purification

Purified by antigen affinity column.

Numéro d'accès

P53990

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

IST1 homolog is a ~39 kDa protein. ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2.

1. Bajorek, M. et al. (2009) Molecular biology of the cell 20, 1360-73. PMID: 19129479

2. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712

3. Gu, M. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2166-E2175. PMID: 28242692

4. Agromayor, M. et al. (2009) Molecular biology of the cell 20, 1374-87. PMID: 19129480

5. Allison, R. et al. (2013) The Journal of cell biology 202, 527-43. PMID: 23897888

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human IST1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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