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| カタログ番号 | HS782014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human KLHL22 (Asp296-Thr592). | ||||||||
| ターゲット | Kelch-like protein 22, KLHL22 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q53GT1 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Kelch-like protein 22 (KLHL22) is a ~71 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. 1. Maerki, S. et al. (2009) The Journal of cell biology 187, 791-800. PMID: 19995937 2. Beck, J. et al. (2013) Nature cell biology 15, 430-9. PMID: 23455478 3. Chen, J. et al. (2018) Nature 557, 585-589. PMID: 29769719 | ||||||||
| 注意事項 | For research use only. |
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