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| カタログ番号 | HA118014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human KLHL3 Polyclonal Antibody (HA118014) is a rabbit polyclonal antibody detecting KLHL3 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human KLHL3 (Thr27-Lys292). | ||||||||
| ターゲット | Kelch-like protein 3, KLHL3, KIAA1129 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9UH77 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Kelch-like protein 3 (KLHL3) is a ~64 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption. The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3. The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation. 1. Furukawa, M. et al. (2003) Nature cell biology 5, 1001-7. PMID: 14528312 2. Louis-Dit-Picard, H. et al. (2012) Nature genetics 44, 456-60, S1-3. PMID: 22406640 3. Ohta, A. et al. (2013) The Biochemical journal 451, 111-22. PMID: 23387299 4. Wakabayashi, M. et al. (2013) Cell reports 3, 858-68. PMID: 23453970 5. Shibata, S. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7838-43. PMID: 23576762 6. Wu, G. et al. (2013) FEBS letters 587, 1717-22. PMID: 23665031 7. Shibata, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 15556-61. PMID: 25313067 8. Etges, A. et al. (2022) Nephron 146, 418-428. PMID: 35093948 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-KLHL3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse liver lysate
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