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Anti-Human KMT5A Polyclonal Antibody (HP554014)

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Número de catálogoHP554014
Descripción
Anti-Human KMT5A Polyclonal Antibody (HP554014) is a rabbit polyclonal antibody detecting KMT5A in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human KMT5A (Leu263-His393).
Objetivo Histone-lysine N-methyltransferase KMT5A, PR/SET07, SETD8, SET8, PR-Set7, Lysine N-methyltransferase 5A, PRSET7, N-lysine methyltransferase KMT5A, SET domain-containing protein 8, Lysine-specific methylase 5A, H4-K20-HMTase KMT5A, SET07, KMT5A, PR/SET domain-containing protein 07
Purificación Purified by antigen affinity column.
Número de acceso Q9NQR1
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

N-lysine methyltransferase KMT5A is a ~42 kDa protein. Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis.

1. Nishioka, K. et al. (2002) Molecular cell 9, 1201-13. PMID: 12086618
2. Fang, J. et al. (2002) Current biology : CB 12, 1086-99. PMID: 12121615
3. Yin, Y. et al. (2005) The Journal of biological chemistry 280, 30025-31. PMID: 15964846
4. Shi, X. et al. (2007) Molecular cell 27, 636-46. PMID: 17707234
5. Saredi, G. et al. (2016) Nature 534, 714-718. PMID: 27338793
6. Julien, E. et al. (2004) Molecular cell 14, 713-25. PMID: 15200950
7. Xiao, B. et al. (2005) Genes & development 19, 1444-54. PMID: 15933069
8. Couture, JF. et al. (2005) Genes & development 19, 1455-65. PMID: 15933070
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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