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Anti-Human Lambda light chain Polyclonal Antibody (HA011014)

Anti-Human Lambda light chain Polyclonal Antibody
Anti-Human Lambda light chain Polyclonal Antibody
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Vista general
Número de catálogoHA011014
Descripción
Anti-Human Lambda light chain Polyclonal Antibody (HA011014) is a rabbit polyclonal antibody detecting Ig lambda-1 chain C region in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human IGLC1 (Gly1-Thr95).
Objetivo Immunoglobulin lambda constant 1, Ig lambda chain C region MGC, Ig lambda-1 chain C region, IGLC1
Purificación Purified by antigen affinity column.
Número de acceso P0CG04
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Immunoglobulin lambda constant 1 (IGLC1) is a ~11 kDa protein. Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
Nota For research use only.
Imágenes
  • Anti-Human Lambda light chain Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-IGLC1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse serum lysate
    Lane 2: Rat thymus lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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