Anti-Human LARP7 Polyclonal Antibody (HS837014)

Name: Anti-Human LARP7 Polyclonal Antibody
Brand: abinScience
SKU: HS837014
Price: 168 USD
Availability: InStock

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100ug

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SDS

Aperçu

Numéro de catalogue

HS837014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human LARP7 (Met1-Asn187).

Cible

La ribonucleoprotein domain family member 7, La-related protein 7, hLARP7, P-TEFb-interaction protein for 7SK stability, LARP7, PIP7S

Purification

Purified by antigen affinity column.

Numéro d'accès

Q4G0J3

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

La-related protein 7 (LARP7) is a ~66 kDa protein. RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function. Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II. The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA; on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex.

1. He, N. et al. (2008) Molecular cell 29, 588-99. PMID: 18249148

2. Hasler, D. et al. (2020) Molecular cell 77, 1014-1031.e13. PMID: 32017898

3. Markert, A. et al. (2008) EMBO reports 9, 569-75. PMID: 18483487

4. Egloff, S. et al. (2017) The EMBO journal 36, 934-948. PMID: 28254838

5. Krueger, BJ. et al. (2008) Nucleic acids research 36, 2219-29. PMID: 18281698

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human LARP7 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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