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Anti-Human LCT Polyclonal Antibody (HY498014)

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Vista general
Número de catálogoHY498014
Descripción
Anti-Human LCT Polyclonal Antibody (HY498014) is a rabbit polyclonal antibody detecting LCT in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human LCT (Ser20-Gly336).
Objetivo Lactase/phlorizin hydrolase, Lactase/glycosylceramidase, Lactase, 3.2.1.108, Glycosylceramidase, 3.2.1.62, Phlorizin hydrolase, LCT, LPH
Purificación Purified by antigen affinity column.
Número de acceso P09848
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Lactase/phlorizin hydrolase (LCT) is a ~218 kDa protein. Broad specificity glycosidase of the intestinal brush border membrane that hydrolyzes lactose, the main sugar in mammalian milk, to produce D-glucose and D-galactose. The mature protein is composed of two domains that catalyze the hydrolysis of beta-glucopyranosides and beta-galactopyranosides, with a preference for hydrophilic aglycones (in lactose and cellobiose) for one domain and hydrophobic aglycones (in phlorizin and glycosylceramides) for the other.

1. Németh, K. et al. (2003) European journal of nutrition 42, 29-42. PMID: 12594539
2. Kuokkanen, M. et al. (2006) American journal of human genetics 78, 339-44. PMID: 16400612
3. Potter, J. et al. (1985) Biochemical genetics 23, 423-39. PMID: 3929764
4. Zecca, L. et al. (1998) FEBS letters 435, 225-8. PMID: 9762914
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human LCT Polyclonal Antibody

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