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Anti-Human LCT Polyclonal Antibody (HY498014)

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Aperçu
Numéro de catalogueHY498014
Description
Anti-Human LCT Polyclonal Antibody (HY498014) is a rabbit polyclonal antibody detecting LCT in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human LCT (Ser20-Gly336).
Cible Lactase/phlorizin hydrolase, Lactase/glycosylceramidase, Lactase, 3.2.1.108, Glycosylceramidase, 3.2.1.62, Phlorizin hydrolase, LCT, LPH
Purification Purified by antigen affinity column.
Numéro d'accès P09848
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Lactase/phlorizin hydrolase (LCT) is a ~218 kDa protein. Broad specificity glycosidase of the intestinal brush border membrane that hydrolyzes lactose, the main sugar in mammalian milk, to produce D-glucose and D-galactose. The mature protein is composed of two domains that catalyze the hydrolysis of beta-glucopyranosides and beta-galactopyranosides, with a preference for hydrophilic aglycones (in lactose and cellobiose) for one domain and hydrophobic aglycones (in phlorizin and glycosylceramides) for the other.

1. Németh, K. et al. (2003) European journal of nutrition 42, 29-42. PMID: 12594539
2. Kuokkanen, M. et al. (2006) American journal of human genetics 78, 339-44. PMID: 16400612
3. Potter, J. et al. (1985) Biochemical genetics 23, 423-39. PMID: 3929764
4. Zecca, L. et al. (1998) FEBS letters 435, 225-8. PMID: 9762914
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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