Anti-Human LIG4 Polyclonal Antibody (HW824014)

Name: Anti-Human LIG4 Polyclonal Antibody
Brand: abinScience
SKU: HW824014
Price: 168 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) : --

Spécification :

50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW824014

Description

Anti-Human LIG4 Polyclonal Antibody (HW824014) is a rabbit polyclonal antibody detecting LIG4 in ELISA, IHC, WB. Suitable for Human, Mouse, Cricetulus griseus, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human LIG4 (Met1-Lys609).

Cible

DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4

Purification

Purified by antigen affinity column.

Numéro d'accès

P49917

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA ligase 4 (LIG4) is a ~103 kDa protein. DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4.

1. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771

2. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226

3. Ochi, T. et al. (2013) Structure (London, England : 1993) 21, 672-9. PMID: 23523427

4. Kaminski, AM. et al. (2018) Nature communications 9, 2642. PMID: 29980672

5. Maddi, ER. et al. (2021) Protein engineering, design & selection : PEDS 34. PMID: 33586762

6. Robins, P. et al. (1996) The Journal of biological chemistry 271, 24257-61. PMID: 8798671

7. Grawunder, U. et al. (1997) Nature 388, 492-5. PMID: 9242410

8. Grawunder, U. et al. (1998) Molecular cell 2, 477-84. PMID: 9809069

9. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human LIG4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human LIG4 Polyclonal Antibody (HW824014)

Aperçu

Images

Reférences

Fiche technique

SDS