Anti-Human LIG4 Polyclonal Antibody (HW824014)

Name: Anti-Human LIG4 Polyclonal Antibody
Brand: abinScience
SKU: HW824014
Price: 168 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HW824014

説明

Anti-Human LIG4 Polyclonal Antibody (HW824014) is a rabbit polyclonal antibody detecting LIG4 in ELISA, IHC, WB. Suitable for Human, Mouse, Cricetulus griseus, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human LIG4 (Met1-Lys609).

ターゲット

DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4

精製

Purified by antigen affinity column.

アクセッション番号

P49917

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA ligase 4 (LIG4) is a ~103 kDa protein. DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4.

1. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771

2. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226

3. Ochi, T. et al. (2013) Structure (London, England : 1993) 21, 672-9. PMID: 23523427

4. Kaminski, AM. et al. (2018) Nature communications 9, 2642. PMID: 29980672

5. Maddi, ER. et al. (2021) Protein engineering, design & selection : PEDS 34. PMID: 33586762

6. Robins, P. et al. (1996) The Journal of biological chemistry 271, 24257-61. PMID: 8798671

7. Grawunder, U. et al. (1997) Nature 388, 492-5. PMID: 9242410

8. Grawunder, U. et al. (1998) Molecular cell 2, 477-84. PMID: 9809069

9. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-Human LIG4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human LIG4 Polyclonal Antibody (HW824014)

概要

イメージ

参考文献

データシート

SDS