Anti-Human LIMK1 Polyclonal Antibody (HW511014)

Name: Anti-Human LIMK1 Polyclonal Antibody
Brand: abinScience
SKU: HW511014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW511014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human LIMK1 (Gly419-Asp647).

Objetivo

LIMK-1, LIMK, LIMK1, LIM domain kinase 1

Purificación

Purified by antigen affinity column.

Número de acceso

P53667

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

LIM domain kinase 1 (LIMK1) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Niwa, R. et al. (2002) Cell 108, 233-46. PMID: 11832213

3. Kaji, N. et al. (2003) The Journal of biological chemistry 278, 33450-5. PMID: 12807904

4. Soosairajah, J. et al. (2005) The EMBO journal 24, 473-86. PMID: 15660133

5. Nishita, M. et al. (2005) The Journal of cell biology 171, 349-59. PMID: 16230460

6. Acevedo, K. et al. (2007) Experimental cell research 313, 4091-106. PMID: 18028908

7. Heng, YW. et al. (2012) Journal of cell science 125, 1579-90. PMID: 22328514

8. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human LIMK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo