Anti-Human LIMK1 Polyclonal Antibody (HW511014)

Name: Anti-Human LIMK1 Polyclonal Antibody
Brand: abinScience
SKU: HW511014
Price: 168 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug

Nombre :

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HW511014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human LIMK1 (Gly419-Asp647).

Cible

LIMK-1, LIMK, LIMK1, LIM domain kinase 1

Purification

Purified by antigen affinity column.

Numéro d'accès

P53667

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

LIM domain kinase 1 (LIMK1) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Niwa, R. et al. (2002) Cell 108, 233-46. PMID: 11832213

3. Kaji, N. et al. (2003) The Journal of biological chemistry 278, 33450-5. PMID: 12807904

4. Soosairajah, J. et al. (2005) The EMBO journal 24, 473-86. PMID: 15660133

5. Nishita, M. et al. (2005) The Journal of cell biology 171, 349-59. PMID: 16230460

6. Acevedo, K. et al. (2007) Experimental cell research 313, 4091-106. PMID: 18028908

7. Heng, YW. et al. (2012) Journal of cell science 125, 1579-90. PMID: 22328514

8. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human LIMK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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