Anti-Human LIMK1 Polyclonal Antibody (HW511014)

Name: Anti-Human LIMK1 Polyclonal Antibody
Brand: abinScience
SKU: HW511014
Price: 168 USD
Availability: InStock

データシート製品共有情報コピー

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100ug

数量:

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HW511014

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human LIMK1 (Gly419-Asp647).

ターゲット

LIMK-1, LIMK, LIMK1, LIM domain kinase 1

精製

Purified by antigen affinity column.

アクセッション番号

P53667

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

LIM domain kinase 1 (LIMK1) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Niwa, R. et al. (2002) Cell 108, 233-46. PMID: 11832213

3. Kaji, N. et al. (2003) The Journal of biological chemistry 278, 33450-5. PMID: 12807904

4. Soosairajah, J. et al. (2005) The EMBO journal 24, 473-86. PMID: 15660133

5. Nishita, M. et al. (2005) The Journal of cell biology 171, 349-59. PMID: 16230460

6. Acevedo, K. et al. (2007) Experimental cell research 313, 4091-106. PMID: 18028908

7. Heng, YW. et al. (2012) Journal of cell science 125, 1579-90. PMID: 22328514

8. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-Human LIMK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human LIMK1 Polyclonal Antibody (HW511014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ