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Anti-Human LIN28A Polyclonal Antibody (HP172014)

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Vista general
Número de catálogoHP172014
Descripción
Anti-Human LIN28A Polyclonal Antibody (HP172014) is a rabbit polyclonal antibody detecting LIN28A in ELISA, IHC, WB. Suitable for Human, Mouse, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human LIN28A (Gln36-Gly111).
Objetivo CSDD1, Zinc finger CCHC domain-containing protein 1, LIN28, LIN28A, Lin-28A, Protein lin-28 homolog A, ZCCHC1
Purificación Purified by antigen affinity column.
Número de acceso Q9H9Z2
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Protein lin-28 homolog A (LIN28A) is a ~22 kDa protein. RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA.

1. Jin, J. et al. (2011) Nucleic acids research 39, 3724-34. PMID: 21247876
2. Heo, I. et al. (2008) Molecular cell 32, 276-84. PMID: 18951094
3. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396
4. Piskounova, E. et al. (2011) Cell 147, 1066-79. PMID: 22118463
5. Thornton, JE. et al. (2012) RNA (New York, N.Y.) 18, 1875-85. PMID: 22898984
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human LIN28A Polyclonal Antibody

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