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Anti-Human LIN28A Polyclonal Antibody (HP172014)

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Aperçu
Numéro de catalogueHP172014
Description
Anti-Human LIN28A Polyclonal Antibody (HP172014) is a rabbit polyclonal antibody detecting LIN28A in ELISA, IHC, WB. Suitable for Human, Mouse, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human LIN28A (Gln36-Gly111).
Cible CSDD1, Zinc finger CCHC domain-containing protein 1, LIN28, LIN28A, Lin-28A, Protein lin-28 homolog A, ZCCHC1
Purification Purified by antigen affinity column.
Numéro d'accès Q9H9Z2
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein lin-28 homolog A (LIN28A) is a ~22 kDa protein. RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA.

1. Jin, J. et al. (2011) Nucleic acids research 39, 3724-34. PMID: 21247876
2. Heo, I. et al. (2008) Molecular cell 32, 276-84. PMID: 18951094
3. Heo, I. et al. (2009) Cell 138, 696-708. PMID: 19703396
4. Piskounova, E. et al. (2011) Cell 147, 1066-79. PMID: 22118463
5. Thornton, JE. et al. (2012) RNA (New York, N.Y.) 18, 1875-85. PMID: 22898984
Note For research use only.
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Reférences
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human LIN28A Polyclonal Antibody

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