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Anti-Human LYAR Polyclonal Antibody (HV132014)

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Vista general
Número de catálogoHV132014
Descripción
Anti-Human LYAR Polyclonal Antibody (HV132014) is a rabbit polyclonal antibody detecting LYAR in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human LYAR (Gly307-Lys379).
Objetivo Cell growth-regulating nucleolar protein, LYAR
Purificación Purified by antigen affinity column.
Número de acceso Q9NX58
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Cell growth-regulating nucleolar protein (LYAR) is a ~43 kDa protein. Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'.

1. Miyazawa, N. et al. (2014) Genes to cells : devoted to molecular & cellular mechanisms 19, 273-86. PMID: 24495227
2. Ju, J. et al. (2014) Nucleic acids research 42, 9740-52. PMID: 25092918
3. Sun, Y. et al. (2017) Cell death and differentiation 24, 1645-1654. PMID: 28686580
4. Yang, C. et al. (2019) Journal of virology 93. PMID: 31413131
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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