Anti-Human LYPLA1 Polyclonal Antibody (HF852014)

Name: Anti-Human LYPLA1 Polyclonal Antibody
Brand: abinScience
SKU: HF852014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HF852014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human LYPLA1 (Met1-Asp230).

Cible

APT1, LYPLA1, LPL-I, Lysophospholipase I, LysoPLA I, LPL1, hAPT1, Acyl-protein thioesterase 1, APT-1, Lysophospholipase 1

Purification

Purified by antigen affinity column.

Numéro d'accès

O75608

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Acyl-protein thioesterase 1 (LYPLA1) is a ~24 kDa protein. Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC).

1. Hirano, T. et al. (2009) Biochimica et biophysica acta 1791, 797-805. PMID: 19439193

2. Dekker, FJ. et al. (2010) Nature chemical biology 6, 449-56. PMID: 20418879

3. Tian, L. et al. (2012) The Journal of biological chemistry 287, 14718-25. PMID: 22399288

4. Adachi, N. et al. (2016) The Journal of biological chemistry 291, 20232-46. PMID: 27481942

5. Huang, X. et al. (2023) Molecular cell 83, 3485-3501.e11. PMID: 37802024

6. Bolen, AL. et al. (2011) Journal of lipid research 52, 958-70. PMID: 21393252

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LYPLA1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate
Lane 2: Mouse kidney lysate
Lane 3: Rat liver lysate
Lane 4: Rat kidney lysate

Reférences

Fiche technique

Anti-Human LYPLA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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