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Anti-Human MAD1L1 Polyclonal Antibody (HP245014)

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Aperçu
Numéro de catalogueHP245014
Description
Anti-Human MAD1L1 Polyclonal Antibody (HP245014) is a rabbit polyclonal antibody detecting MAD1L1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human MAD1L1 (His544-Ala718).
Cible HsMAD1, MAD1-like protein 1, hMAD1, Mitotic arrest deficient 1-like protein 1, Tax-binding protein 181, MAD1, TXBP181, Mitotic checkpoint MAD1 protein homolog, MAD1L1, Mitotic spindle assembly checkpoint protein MAD1
Purification Purified by antigen affinity column.
Numéro d'accès Q9Y6D9
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Mitotic spindle assembly checkpoint protein MAD1 (MAD1L1) is a ~83 kDa protein. Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling.

1. Jin, DY. et al. (1999) Genomics 55, 363-4. PMID: 10049595
2. Nakano, H. et al. (2010) The Journal of biological chemistry 285, 10841-9. PMID: 20133940
3. Ji, W. et al. (2018) The Journal of biological chemistry 293, 484-496. PMID: 29162720
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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