Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

Name: Anti-Human MAD2L2 Polyclonal Antibody
Brand: abinScience
SKU: HP994014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Vista general

Número de catálogo

HP994014

Descripción

Anti-Human MAD2L2 Polyclonal Antibody (HP994014) is a rabbit polyclonal antibody detecting MAD2L2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human MAD2L2 (Met1-Ser211).

Objetivo

Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B, MAD2L2, MAD2-like protein 2, hREV7, REV7, REV7 homolog, MAD2B

Purificación

Purified by antigen affinity column.

Número de acceso

Q9UI95

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) is a ~24 kDa protein. Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection.

1. Pfleger, CM. et al. (2001) Genes & development 15, 1759-64. PMID: 11459825

2. Chen, J. et al. (2001) Genes & development 15, 1765-70. PMID: 11459826

3. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

4. Iwai, H. et al. (2007) Cell 130, 611-23. PMID: 17719540

5. Hong, CF. et al. (2009) The Journal of biological chemistry 284, 19613-22. PMID: 19443654

6. Gupta, R. et al. (2018) Cell 173, 972-988.e23. PMID: 29656893

7. Hara, K. et al. (2010) The Journal of biological chemistry 285, 12299-307. PMID: 20164194

8. Xie, W. et al. (2012) Protein & cell 3, 864-74. PMID: 23143872

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-MAD2L2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate

Referencias

Datasheet

Anti-Human MAD2L2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo