Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

Name: Anti-Human MAD2L2 Polyclonal Antibody
Brand: abinScience
SKU: HP994014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HP994014

Описание

Anti-Human MAD2L2 Polyclonal Antibody (HP994014) is a rabbit polyclonal antibody detecting MAD2L2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human MAD2L2 (Met1-Ser211).

Цель

Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B, MAD2L2, MAD2-like protein 2, hREV7, REV7, REV7 homolog, MAD2B

Очистка

Purified by antigen affinity column.

Номер доступа

Q9UI95

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) is a ~24 kDa protein. Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection.

1. Pfleger, CM. et al. (2001) Genes & development 15, 1759-64. PMID: 11459825

2. Chen, J. et al. (2001) Genes & development 15, 1765-70. PMID: 11459826

3. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

4. Iwai, H. et al. (2007) Cell 130, 611-23. PMID: 17719540

5. Hong, CF. et al. (2009) The Journal of biological chemistry 284, 19613-22. PMID: 19443654

6. Gupta, R. et al. (2018) Cell 173, 972-988.e23. PMID: 29656893

7. Hara, K. et al. (2010) The Journal of biological chemistry 285, 12299-307. PMID: 20164194

8. Xie, W. et al. (2012) Protein & cell 3, 864-74. PMID: 23143872

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-MAD2L2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate

Ссылки

Даташит

Anti-Human MAD2L2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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