Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Name: Anti-Human MAGEA3 Recombinant Antibody (SAA1562)
Brand: abinScience
SKU: HW798013
Price: 110 USD
Availability: InStock

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20ug
50ug
100ug
1mg

Número:

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Datasheet
SDS

Vista general

Número de catálogo

HW798013

Descripción

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013) is a mouse monoclonal antibody detecting MAGEA3 in ELISA, FCM. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, FCM

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a, kappa

ID de clon

SAA1562

Aplicaciones probadas

FCM

Objetivo

Antigen MZ2-D, MAGE3, Melanoma-associated antigen 3, CT1.3, MAGE-3 antigen, Cancer/testis antigen 1.3, MAGEA3

Nivel de endotoxinas

< 10 EU/mg as determined by LAL method.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P43357

RRID

Anti-Human MAGEA3 Antibody (SAA1562) (abinScience Cat# HW798013, RRID:AB_3727323)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Melanoma-associated antigen 3 (MAGEA3) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

1. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041

2. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705

3. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928

4. Gaugler, B. et al. (1994) The Journal of experimental medicine 179, 921-30. PMID: 8113684

Nota

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human MAGEA3 Antibody (SAA1562)
Flow cytometry

Flow-cytometry using anti-human MAGEA3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MAGEA3 monoclonal antibody (Catalog HW798013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-Human MAGEA3 Recombinant Antibody (SAA1562).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Vista general

Imágenes

Referencias

Datasheet

SDS