Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Name: Anti-Human MAGEA3 Recombinant Antibody (SAA1562)
Brand: abinScience
SKU: HW798013
Price: 110 USD
Availability: InStock

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20ug
50ug
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1mg

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Aperçu

Numéro de catalogue

HW798013

Description

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013) is a mouse monoclonal antibody detecting MAGEA3 in ELISA, FCM. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, FCM

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

SAA1562

Applications testées

FCM

Cible

Antigen MZ2-D, MAGE3, Melanoma-associated antigen 3, CT1.3, MAGE-3 antigen, Cancer/testis antigen 1.3, MAGEA3

Niveau d'endotoxines

< 10 EU/mg as determined by LAL method.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P43357

RRID

Anti-Human MAGEA3 Antibody (SAA1562) (abinScience Cat# HW798013, RRID:AB_3727323)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Melanoma-associated antigen 3 (MAGEA3) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

1. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041

2. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705

3. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928

4. Gaugler, B. et al. (1994) The Journal of experimental medicine 179, 921-30. PMID: 8113684

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

SDS-PAGE

SDS-PAGE for Anti-Human MAGEA3 Antibody (SAA1562)
Flow cytometry

Flow-cytometry using anti-human MAGEA3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MAGEA3 monoclonal antibody (Catalog HW798013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences

Fiche technique

Anti-Human MAGEA3 Recombinant Antibody (SAA1562).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Aperçu

Images

Reférences

Fiche technique

SDS