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| カタログ番号 | HW798013 | ||||
|---|---|---|---|---|---|
| 説明 |
Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013) is a mouse monoclonal antibody detecting MAGEA3 in ELISA, FCM. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||
| アプリケーション | ELISA, FCM | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a, kappa | ||||
| クローンID | SAA1562 | ||||
| テスト済みアプリケーション | FCM | ||||
| ターゲット | Antigen MZ2-D, MAGE3, Melanoma-associated antigen 3, CT1.3, MAGE-3 antigen, Cancer/testis antigen 1.3, MAGEA3 | ||||
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P43357 | ||||
| RRID | Anti-Human MAGEA3 Antibody (SAA1562) (abinScience Cat# HW798013, RRID:AB_3727323) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Melanoma-associated antigen 3 (MAGEA3) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. 1. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041 2. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705 3. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928 4. Gaugler, B. et al. (1994) The Journal of experimental medicine 179, 921-30. PMID: 8113684 | ||||
| 注意事項 | For research use only. | ||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
SDS-PAGE for Anti-Human MAGEA3 Antibody (SAA1562)
Flow-cytometry using anti-human MAGEA3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MAGEA3 monoclonal antibody (Catalog HW798013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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