Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Name: Anti-Human MAGEA3 Recombinant Antibody (SAA1562)
Brand: abinScience
SKU: HW798013
Price: 110 USD
Availability: InStock

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Спецификация:

20ug
50ug
100ug
1mg

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Обзор

Каталожный номер

HW798013

Описание

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013) is a mouse monoclonal antibody detecting MAGEA3 in ELISA, FCM. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human

Применения

ELISA, FCM

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a, kappa

ID клона

SAA1562

Проверенные приложения

FCM

Цель

Antigen MZ2-D, MAGE3, Melanoma-associated antigen 3, CT1.3, MAGE-3 antigen, Cancer/testis antigen 1.3, MAGEA3

Уровень эндотоксинов

< 10 EU/mg as determined by LAL method.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P43357

RRID

Anti-Human MAGEA3 Antibody (SAA1562) (abinScience Cat# HW798013, RRID:AB_3727323)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Melanoma-associated antigen 3 (MAGEA3) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

1. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041

2. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705

3. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928

4. Gaugler, B. et al. (1994) The Journal of experimental medicine 179, 921-30. PMID: 8113684

Примечание

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Изображения

SDS-PAGE

SDS-PAGE for Anti-Human MAGEA3 Antibody (SAA1562)
Flow cytometry

Flow-cytometry using anti-human MAGEA3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MAGEA3 monoclonal antibody (Catalog HW798013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Ссылки

Даташит

Anti-Human MAGEA3 Recombinant Antibody (SAA1562).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

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