Anti-Human MAGOH Polyclonal Antibody (HX135014)

Name: Anti-Human MAGOH Polyclonal Antibody
Brand: abinScience
SKU: HX135014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Vista general

Número de catálogo

HX135014

Descripción

Anti-Human MAGOH Polyclonal Antibody (HX135014) is a rabbit polyclonal antibody detecting MAGOH in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human MAGOH (Met1-Ile146).

Objetivo

MAGOH, Protein mago nashi homolog, MAGOHA

Purificación

Purified by antigen affinity column.

Número de acceso

P61326

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Protein mago nashi homolog (MAGOH) is a ~17 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638

2. Singh, KK. et al. (2013) RNA biology 10, 1291-8. PMID: 23917022

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-MAGOH polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HL60 cell lysate
Lane 3: K562 cell lysate
Lane 4: Raji cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Lncap cell lysate

Referencias

Datasheet

Anti-Human MAGOH Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human MAGOH Polyclonal Antibody (HX135014)

Vista general

Imágenes

Referencias

Datasheet

SDS