中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HX135014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human MAGOH Polyclonal Antibody (HX135014) is a rabbit polyclonal antibody detecting MAGOH in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
|
||||||||
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MAGOH (Met1-Ile146). | ||||||||
| ターゲット | MAGOH, Protein mago nashi homolog, MAGOHA | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P61326 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Protein mago nashi homolog (MAGOH) is a ~17 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. 1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638 2. Singh, KK. et al. (2013) RNA biology 10, 1291-8. PMID: 23917022 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-MAGOH polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HL60 cell lysate
Lane 3: K562 cell lysate
Lane 4: Raji cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Lncap cell lysate
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
