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Anti-Human Tau Recombinant Antibody (MC1)
Anti-Human Tau Recombinant Antibody (MC1)
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
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Обзор
Каталожный номерHY086203
Реактивность видовHuman
ПримененияELISA, FCM
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипIgG2a, kappa
ID клонаMC1
Проверенные приложения FCM
Цель MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU
Уровень эндотоксинов < 10 EU/mg
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P10636
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Microtubule-associated protein tau (MAPT) is a ~78 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

1. Yoshida, H. et al. (2012) Journal of neurochemistry 120, 165-76. PMID: 21985311
2. Sandberg, A. et al. (2020) Neurobiology of disease 146, 105079. PMID: 32961270
Примечание For research use only.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Изображения
  • Anti-Human Tau Recombinant Antibody (MC1)

    Flow CytoMetry

    Flow-cytometry using FITC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human MAPT monoclonal antibody (Catalog: HY086203, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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