Anti-Human MARCHF8 Polyclonal Antibody (HP368014)

Name: Anti-Human MARCHF8 Polyclonal Antibody
Brand: abinScience
SKU: HP368014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HP368014

Description

Anti-Human MARCHF8 Polyclonal Antibody (HP368014) is a rabbit polyclonal antibody detecting MARCHF8 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MARCHF8 (Ser13-Lys137).

Cible

Cellular modulator of immune recognition, E3 ubiquitin-protein ligase MARCHF8, MARCH-VIII, MIR, RING finger protein 178, MARCHF8, Membrane-associated RING-CH protein VIII, c-MIR, MARCH8, Membrane-associated RING finger protein 8, RNF178, RING-type E3 ubiquitin transferase MARCHF8

Purification

Purified by antigen affinity column.

Numéro d'accès

Q5T0T0

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

E3 ubiquitin-protein ligase MARCHF8 is a ~32 kDa protein. E3 ubiquitin-protein ligase that plays several important roles in innate immunity and adaptive immunity. Mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. Possesses a very broad antiviral activity by specifically inactivating different viral fusion proteins. Targets and ubiquitinates cytoplasmic lysine residues of viral envelope glycoproteins with single transmembrane domains leading to their lysosomal degradation. Therefore, shows broad-spectrum inhibition against many viruses including retroviruses, rhabdoviruses, arenaviruses, sarbecoviruses or influenzaviruses.

1. Liu, X. et al. (2021) Nature communications 12, 4427. PMID: 34285233

2. Zhang, Y. et al. (2022) Microbiology spectrum 10, e0061821. PMID: 35019698

3. Yang, X. et al. (2022) Science signaling 15, eabk3067. PMID: 35503863

4. Lapaque, N. et al. (2009) The Journal of biological chemistry 284, 7007-16. PMID: 19117940

5. Gauvreau, ME. et al. (2009) Traffic (Copenhagen, Denmark) 10, 1518-27. PMID: 19566897

6. Yu, C. et al. (2020) mBio 11. PMID: 32934085

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human MARCHF8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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