Anti-Human MBD3 Polyclonal Antibody (HF905014)

Name: Anti-Human MBD3 Polyclonal Antibody
Brand: abinScience
SKU: HF905014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HF905014

説明

Anti-Human MBD3 Polyclonal Antibody (HF905014) is a rabbit polyclonal antibody detecting MBD3 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human MBD3 (Lys4-Glu253).

ターゲット

Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, MBD3

精製

Purified by antigen affinity column.

アクセッション番号

O95983

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Methyl-CpG-binding domain protein 3 (MBD3) is a ~32 kDa protein. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to methylated DNA by itself. Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.

1. Saito, M. et al. (2002) The Journal of biological chemistry 277, 35434-9. PMID: 12124384

2. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

3. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

4. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852

5. Morey, L. et al. (2008) Molecular and cellular biology 28, 5912-23. PMID: 18644863

6. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-Human MBD3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human MBD3 Polyclonal Antibody (HF905014)

概要

イメージ

参考文献

データシート

SDS