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Обзор
Каталожный номерHF905014
Описание
Anti-Human MBD3 Polyclonal Antibody (HF905014) is a rabbit polyclonal antibody detecting MBD3 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human MBD3 (Lys4-Glu253).
Цель Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, MBD3
Очистка Purified by antigen affinity column.
Номер доступа O95983
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Methyl-CpG-binding domain protein 3 (MBD3) is a ~32 kDa protein. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to methylated DNA by itself. Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.

1. Saito, M. et al. (2002) The Journal of biological chemistry 277, 35434-9. PMID: 12124384
2. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440
3. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666
4. Tatematsu, KI. et al. (2000) Genes to cells : devoted to molecular & cellular mechanisms 5, 677-88. PMID: 10947852
5. Morey, L. et al. (2008) Molecular and cellular biology 28, 5912-23. PMID: 18644863
6. Cramer, JM. et al. (2014) The Journal of biological chemistry 289, 1294-302. PMID: 24307175
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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