Anti-Human MBTD1 IP Recombinant Antibody (SAA0210) (HC542013)

Name: Anti-Human MBTD1 IP Recombinant Antibody (SAA0210)
Brand: abinScience
SKU: HC542013
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HC542013

Reactividad de especies

Human

Aplicaciones

ELISA, IF, IP, IP-MS

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG

ID de clon

SAA0210

Objetivo

MBTD1, MBT domain-containing protein 1

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q05BQ5

RRID

Anti-Human MBTD1 IP Antibody (SAA0210) (abinScience Cat# HC542013, RRID:AB_3729744)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

MBT domain-containing protein 1 (MBTD1) is a ~70 kDa protein. Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me. Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR).

1. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

2. Zhang, H. et al. (2020) Cell reports 30, 3996-4002.e4. PMID: 32209463

3. Eryilmaz, J. et al. (2009) PloS one 4, e7274. PMID: 19841675

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human MBTD1 IP Antibody (SAA0210)

Referencias

Datasheet

Anti-Human MBTD1 IP Recombinant Antibody (SAA0210).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo