Anti-Human MBTD1 IP Recombinant Antibody (SAA0210) (HC542013)

Name: Anti-Human MBTD1 IP Recombinant Antibody (SAA0210)
Brand: abinScience
SKU: HC542013
Price: 191 USD
Availability: InStock

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Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC542013

Reactivité des espèces

Human

Applications

ELISA, IF, IP, IP-MS

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG

ID de clone

SAA0210

Cible

MBTD1, MBT domain-containing protein 1

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q05BQ5

RRID

Anti-Human MBTD1 IP Antibody (SAA0210) (abinScience Cat# HC542013, RRID:AB_3729744)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

MBT domain-containing protein 1 (MBTD1) is a ~70 kDa protein. Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me. Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR).

1. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

2. Zhang, H. et al. (2020) Cell reports 30, 3996-4002.e4. PMID: 32209463

3. Eryilmaz, J. et al. (2009) PloS one 4, e7274. PMID: 19841675

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MBTD1 IP Antibody (SAA0210)

Reférences

Fiche technique

Anti-Human MBTD1 IP Recombinant Antibody (SAA0210).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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