Anti-Human MCM6 Polyclonal Antibody (HC528014)

Name: Anti-Human MCM6 Polyclonal Antibody
Brand: abinScience
SKU: HC528014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HC528014

Descripción

Anti-Human MCM6 Polyclonal Antibody (HC528014) is a rabbit polyclonal antibody detecting MCM6 in ELISA, IHC, WB. Suitable for Rat, Bovine, Human, Mouse, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human MCM6 (Arg190-Thr661).

Objetivo

DNA replication licensing factor MCM6, p105MCM, MCM6

Purificación

Purified by antigen affinity column.

Número de acceso

Q14566

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

DNA replication licensing factor MCM6 is a ~92 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

6. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-MCM6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HL60 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: PC12 cell lysate
Lane 6: NIH3T3 cell lysate
Lane 7: Mouse spleen lysate