Anti-Human MCM6 Polyclonal Antibody (HC528014)

Name: Anti-Human MCM6 Polyclonal Antibody
Brand: abinScience
SKU: HC528014
Price: 96 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HC528014

Description

Anti-Human MCM6 Polyclonal Antibody (HC528014) is a rabbit polyclonal antibody detecting MCM6 in ELISA, IHC, WB. Suitable for Rat, Bovine, Human, Mouse, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MCM6 (Arg190-Thr661).

Cible

DNA replication licensing factor MCM6, p105MCM, MCM6

Purification

Purified by antigen affinity column.

Numéro d'accès

Q14566

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

DNA replication licensing factor MCM6 is a ~92 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

6. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MCM6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HL60 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: PC12 cell lysate
Lane 6: NIH3T3 cell lysate
Lane 7: Mouse spleen lysate