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Anti-Human Methionine synthase/MTR Polyclonal Antibody (HC238014)

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Vista general
Número de catálogoHC238014
Descripción
Anti-Human Methionine synthase/MTR Polyclonal Antibody (HC238014) is a rabbit polyclonal antibody detecting Methionine synthase. Suitable for Human and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human Methionine synthase/MTR (Leu924-Asp1265).
Objetivo 5-methyltetrahydrofolate--homocysteine methyltransferase, Cobalamin-dependent methionine synthase, EC:2.1.1.13, MS, MTR, Methionine synthase, Vitamin-B12 dependent methionine synthase
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q99707
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Methionine synthase (MTR) is a ~140 kDa protein. Catalyzes the transfer of a methyl group from methylcob(III)alamin (MeCbl) to homocysteine, yielding enzyme-bound cob(I)alamin and methionine in the cytosol. MeCbl is an active form of cobalamin (vitamin B12) used as a cofactor for methionine biosynthesis. Cob(I)alamin form is regenerated to MeCbl by a transfer of a methyl group from 5-methyltetrahydrofolate. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine.

1. Yamada, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 9476-81. PMID: 16769880
2. Wolthers, KR. et al. (2007) The FEBS journal 274, 738-50. PMID: 17288554
3. Bassila, C. et al. (2017) Biochimica et biophysica acta. Molecular basis of disease 1863, 103-112. PMID: 27771510
Nota For research use only
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human Methionine synthase/MTR Polyclonal Antibody

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