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Anti-Human Methionine synthase/MTR Polyclonal Antibody (HC238014)

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Обзор
Каталожный номерHC238014
Описание
Anti-Human Methionine synthase/MTR Polyclonal Antibody (HC238014) is a rabbit polyclonal antibody detecting Methionine synthase. Suitable for Human and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human Methionine synthase/MTR (Leu924-Asp1265).
Цель 5-methyltetrahydrofolate--homocysteine methyltransferase, Cobalamin-dependent methionine synthase, EC:2.1.1.13, MS, MTR, Methionine synthase, Vitamin-B12 dependent methionine synthase
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q99707
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Фон

Methionine synthase (MTR) is a ~140 kDa protein. Catalyzes the transfer of a methyl group from methylcob(III)alamin (MeCbl) to homocysteine, yielding enzyme-bound cob(I)alamin and methionine in the cytosol. MeCbl is an active form of cobalamin (vitamin B12) used as a cofactor for methionine biosynthesis. Cob(I)alamin form is regenerated to MeCbl by a transfer of a methyl group from 5-methyltetrahydrofolate. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine.

1. Yamada, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 9476-81. PMID: 16769880
2. Wolthers, KR. et al. (2007) The FEBS journal 274, 738-50. PMID: 17288554
3. Bassila, C. et al. (2017) Biochimica et biophysica acta. Molecular basis of disease 1863, 103-112. PMID: 27771510
Примечание For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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